cf 488a carbohydrazide  (Biotium)

Journal: Molecular cell

Article Title: Metabolic environment-driven remodeling of mitochondrial ribosomes regulates translation and biogenesis

doi: 10.1016/j.molcel.2025.10.012

Figure Lengend Snippet: (A–C) Representative images, quantification, and immunoblot of BiG Mito-Split-GFP in cells cultured in glucose medium, with or without co-expression of three MRPs G from the leakage expression of the Z3EV promoter (D-mitoribosome +G cells). (D) Comparison of the oxygen consumption rate (OCR) between wild-type cells and D-mitoribosome +G cells in glucose medium. (E and F) Representative images and quantification of mitochondrial biomass in wild-type cells and D-mitoribosome +G cells cultured in glucose medium. (G) Representative images and quantification of BiG Mito-Split-GFP in different ages of wild-type cells and D-mitoribosome +G cells cultured in YPD. Yeast bud scars (replicative age) were stained by 633 wheat germ agglutinin (WGA). Individual channels of WGA, BiG Mito-Split-GFP, and mito-GFP 11 -mCherry are merged and displayed as a composite. (H) Representative images and quantification of the mitochondrial network size in old wild-type cells and old D-mitoribosome +G cells cultured in glucose medium. Mitochondria were stained by MitoTracker, and bud scars (replicative age) were stained by AlexaFluor 488-WGA. Individual channels of WGA and MitoTracker are merged and displayed as composite. Bar graphs are mean and SEM (cell numbers for each strain refer to ). Scale bar: 5 μm. WT, wild-type cells.

Article Snippet: CF ® 488A Wheat Germ Agglutinin (WGA) , Biotium , 29022-1.

Techniques: Western Blot, Cell Culture, Expressing, Comparison, Staining